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Biosafety Program Environmental Health & Safety

Biosafety Procedure Profile

Step 1 of 6

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Name of Contact and Contact Information:*
First Last
Email address:*
Phone:*
Name of Laboratory PI:*
First Last

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Lab Procedure Title:
The procedure involves the following biological hazards:
(Check all that apply).
- cultures of agents infectious to humans
- human diagnostic specimens that may contain an infectious agent
- human cells
- recombinant or synthetic nucleic acids
- cultures of agents infectious to animals
- animal diagnostic specimens that may contain an infectious agent
- cultures of agents infectious to plants
- plant diagnostic specimens that may contain an infectious agent
- biological toxins
For procedures involving human-derived materials or infectious agents/toxins affecting humans:
Please describe any portions of the process/procedure that have an elevated potential for a potentially infectious materials exposure incident due to the nature of the procedure, the mechanical equipment required or the specified materials being manipulated. With each risk item, please list specific procedural reminders to minimize these exposure risks.
For procedures involving animal or plant materials or infectious agents affecting animals or plants:
Please describe any portions of the process/procedure that have an elevated potential for an environmental release due to the nature of the procedure, the mechanical equipment required or the specific materials being manipulated. With each risk item, please list specific procedural reminders to minimize these environmental release risks. (There are items are likely to be specified on APHIS permits).
Please describe the materials here (strain, source information, etc.):

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Do the exposure risks associated with the process/procedure warrant that lab staff be notified prior to initiating the procedures?
- Yes
- No
Actions to be taken to notify lab staff and restrict traffic flow during the procedure include the following:
Where is this procedure to be carried out? (specify which lab, biological safety cabinet, bench area, etc.)
Does this process/procedure involve the use of sharp devices in conjunction with the potentially infectious materials?
- Yes
- No
What sharp devices are used for this procedure?
(Check all that apply).
- Pasteur Pipettes
- Capillary Tube
- Glass Slides
- Hypodermic Needle
- Scalpel
- Other
Please specify:
Specific sharps safety reminders for this procedure include:

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Personal Protective Equipment (PPE):
- 1. A lab coat and fluid-resistant disposable gloves are to be worn as minimum PPE for ALL procedures that involve handling potentially infectious materials.
- 2. Other PPE to be worn for this process/procedure include the following:
(Check all that apply).
- Double gloves to address high potential for hand contamination
- Impact resistant safety glasses with side shields for bench work outside of a shielded environment (i.e., behind a biological safety cabinet or fume hood sash)
- Splash goggles because the procedure involves liquid quantities and processes that are likely to produce hazardous splashes or sprays outside of a shielded environment (i.e., behind a biological safety cabinet or fume hood sash)
- Face shield to reduce the possibility of skin or mucous membrane contamination that could facilitate lab-acquired infection occurrence
- Surgical mask or N-95 respirator for mouth/nose protection or airway protection respectively. Note: N-95 respirator use is subject to OSHA respiratory protection requirements
- Other
Double gloves to address high potential for hand contamination; this applies to the following procedural steps:
Impact resistant safety glasses with side shields for bench work outside of a shielded environment (i.e., behind a biological safety cabinet or fume hood sash); this applies to the following procedural steps:
Splash goggles because the procedure involves liquid quantities and processes that are likely to produce hazardous splashes or sprays outside of a shielded environment (i.e., behind a BSC or fume hood sash); this applies to the following procedural steps:
Face shield to reduce the possibility of skin or mucous membrane contamination that could facilitate lab-acquired infection occurrence; this applies to the following procedural steps:
Surgical mask or N-95 respirator for mouth/nose protection or airway protection respectively. This applies to the following procedural steps:
Note: N-95 respirator use is subject to OSHA respiratory protection requirements; contact Campus EH&S for additional information at (865) 974-5084.
Other; describe here including rationale and applicable procedural steps:

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Transport/Spill Considerations:
- 1. All containers of potentially infectious materials must be placed in a secondary container that will effectively prevent an environmental release/spill if such containers are to be removed from the BSL-2 lab and be transported through public hallways where food and drink may be present or consumed.
- 2. In the event of a spill of biological materials in the lab, isolate the area, immediately notify others in the labs and your supervisor. Then, take the following actions:
(Check all that apply).
- Retrieve the spill kit and clean up the spill.
- Contact the designated spill responder.
The location of the kit is:
Contact's Name:
First Last
Contact's Phone Number:

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Disinfection:
- 1. All surfaces that may have been contaminated with aerosols or splashes during the manipulation MUST be cleaned of visible contamination AND disinfected at the conclusion of procedures or the end of the workday. This includes bench tops, biological safety cabinets, sample containers, and mechanical equipment used in manipulation and culturing.
- 2. The disinfectant to be used for this process/procedure is:
Dinsinfectant dilution ratio:
Dinsinfectant shelf-life:
Dinsinfectant contact time:
Email
This field is for validation purposes and should be left unchanged.

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Biosafety Program
Environmental Health & Safety

5723 Middlebrook Pike, Suite 119
Knoxville, TN 37921
Brian Ranger, MS, SM (NRCM), CBSP - Biological Safety Officer
Main Phone: 865-974-5084 • Fax: 865-974-0094
Email: utbiosafety@utk.edu

The University of Tennessee, Knoxville

The University of Tennessee, Knoxville
Knoxville, Tennessee 37996
865-974-1000

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