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Standard Microbiological Practices

Who needs this?

This section covers basic safety training for anyone working with non-infectious or low risk (Risk Group 1) microorganisms, diagnostic/environmental samples not known or suspected to contain pathogens, or handling wastes contaminated with such materials.

Who provides training?

It is the responsibility of the Principal Investigator or Lab Supervisor to make sure you have an adequate understanding of Standard Microbiological Practices before working in a lab environment. The Biosafety Office can also provide this training upon request.

How is training given?

Training is provided at the discretion of the Principal Investigator or Lab Supervisor. A Guidance document and training module are available through the Biosafety website. In-person training is also provided by the Biosafety Office upon request. Contact the Biosafety Office at (865) 974-5547 to schedule training.

How frequently must a person be trained?

Training should occur upon entrance to the laboratory environment and retraining should occur anytime there is a change in the lab procedures that impact safe handling practices. Training should be documented and reported to the Biosafety Office.


This training is a recommended resource for anyone working with Biosafety Level-1 organisms or materials.  These would include experiments that do not involve potentially infectious agents or materials.

The following module will review basic good hygiene, safety, and security practices that apply to all biology labs.

Note: There is no online quiz for this module.

After completing the training, follow up with your Laboratory Supervisor for site-specific training.